Assembling Supplies. The most appropriate slide consists of a film that is exactly one cell thick for maximum visualization of all cell types microscopically. You dont need to do anything to prepare for a BNP test or NT-proBNP test. Send whole blood in a tan-top tube. Remove the stopper and carefully aspirate all serum from cells, using a separate disposable pipette for each tube. The film should be free of waves, holes, and ridges, and it should have a smooth appearance and feathered edge. WebB-type Natriuretic Peptide (BNP) Testing. BNP acts as a vasodilator and has diuretic and natriuretic properties. BNP is predominantly secreted by the ventricles of the heart in response to increased pressure. Providing a Specimen. Maisel and coworkers4 found that a BNP cutoff of 75 pg/mL could be used to predict systolic and/or diastolic abnormalities accurately in symptomatic patients referred to echocardiology for left ventricular function studies. Testing schedules may vary. Collect in a plastic lavender-top (EDTA) tube. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample. All microscopic slides, as well as paraffin blocks, should be clearly labeled using two patient identifiers. Use blood transfer device to fill tubes from syringe. Dispose of the used needle and holder in a sharps container according to the provisions in your exposure control plan. Why are my LabCorp results taking so long? 2. Let go. 8. Mix blood with anticoagulant thoroughly, using a rolling wrist motion and by inverting the tube gently 4 or 10 times. Always check the test description to determine whether a gel-barrier tube is acceptable. When performed, it enables the technologist to view the actual physical appearance of the red and white blood cells microscopically. Lott JA, Lu CJ. endstream
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Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. 1-ranked heart program in the United States. Send plasma in a plastic transport tube labeled Plasma, Sodium Heparin or Plasma, Lithium Heparin. Send whole blood in a green-top tube. Specimens for multiple tests should be frozen into different transport tubes. Talk to your provider if you have any questions about the test results. The Role of BNP Testing in Heart Failure. 0000429848 00000 n
testing to when the result is released to the ordering provider. Additional information regarding LOINC codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf. The 8.5 mL red-top tube will yield approximately 3.5 mL serum after clotting and centrifuging. Rumley A, Emberson JR, Wannamethee SG, Lennon L, Whincup PH, Lowe GD. We have methods to check for biotin interference as well as for any other interferences and for the accuracy of the results. 11. Get emergency medical help if you have these symptoms. ), After allowing the clot to form, insert the tube in the centrifuge, stopper end up. 0000585798 00000 n
1. What is the purpose of the BNP test? To address this, the American College of Physicians, based on best available evidence and recent guidelines, recommends that clinicians use age-adjusted D-dimer thresholds in patients greater than 50 years of age with: (a) a low probability of PE who do not meet all "pulmonary-embolism-rule-out criteria," or (b) in those with intermediate probability of PE.21 The formula for an age-adjusted D-dimer cutoff is "age/100." Evaluation of patients with suspected acute pulmonary embolism: Best practice advice from the clinical guidelines committee of the American College of Physicians. This will cause a shift in fluid between the vein and the surrounding tissue. Dao Q, Krishnaswamy P, Kazanegra R, et al. Place the collection slides frosted side up and to your right on a padded, flat surface near the chair or bed where the specimen is to be collected. Fibrin D-dimer and cardiovascular risk. Activate the needle's safety feature and dispose of the needle in a sharps container. Collect in a plastic lavender-top (EDTA) tube. Even though your heart makes this protein, providers sometimes call it brain natriuretic peptide because it was first discovered in brain tissue. 0000148369 00000 n
The Frozen Specimen Keeper will be left in your lockbox for reuse. 0000876850 00000 n
3. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for Plasma Preparation. 49482). A basic metabolic panel (BMP) is a test that measures eight different substances in your blood. This blood test measures the levels of a protein called BNP in your bloodstream. Lott JA, Speicher CE, Nemesnszky E. Is serum amylase an obsolete test in the diagnosis of acute pancreatitis? D-dimer levels are known to increase with age; eg, median D-dimer levels in apparently healthy men aged 75 to 79 are about twice as high as in men aged 60 to 64.19 This increase in the basal D-dimer concentration is responsible for the decrease in the specificity of D-dimer measurements for exclusion of VTE in the elderly. Small amounts of moisture can cause hemolysis. Its important to diagnose heart failure accurately (and rule out other possible conditions) so you can start treatment as soon as possible. 0000581467 00000 n
A small bore, sharp needle causes minimum patient discomfort; 22- or 23-gauge is the smallest bore (or lumen) size recommended to avoid hemolysis. Gumaste VV, Dave PB, Weissman D, Messer J. Lipase:amylase ratio. 0000185379 00000 n
This test is commonly done in the emergency department if you show up with symptoms Do not reuse needles. Royal blue-top tube: Contains sodium EDTA for trace metal studies. Failure to keep the entire edge of the spreader slide against the slide while making the film. 0000222343 00000 n
Considerations for Single and Multiple Sample Collection. To ensure accurate test results, all tubes containing an anticoagulant or preservative must be allowed to fill completely. Handle all slides only by the frosted ends or by the edges. Coming to a Cleveland Clinic location?Hillcrest Cancer Center check-in changesCole Eye entrance closingVisitation and COVID-19 information, Notice of Intelligent Business Solutions data eventLearn more. Log in or register online. The utility of pretest probability assessment in patients with clinically suspected venous thromboembolism. 0000945248 00000 n
Test Tubes Color When multiple tests requiring frozen serum are ordered, a plastic transport tube should be prepared for each test. How long does it take to get blood work back from labcorp? The LOINC codes are copyright 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. 0.5 mL (500 L for pediatric microtainer capillary tubes; fill tube to capacity.) Immediately centrifuge the specimen for as long as 10 minutes or as specified by the tube manufacturer.
Frozen Gel Packs. 0000387970 00000 n
Wu AH, Packer M, Smith A et al. If you have after-hours pickup for frozen specimens, label the tube with a permanent marker. Relationship to infection and sepsis. Draw the spreader slide steadily back toward the drop of blood. 0000006424 00000 n
Storage Instructions Maintain specimen at room temperature. 2021 Laboratory Corporation of America Holdings and Lexi-Comp Inc. All Rights Reserved. This mixed blood may then be centrifuged, yielding plasma, which contains albumin, globulin, and fibrinogen. Carefully push the tube forward until the top edge of the stopper meets the guideline on the holder. If youve received a heart failure diagnosis, there are things you can do to live with heart failure, including lifestyle and dietary changes. But it shouldnt hurt. In: Thomas L, ed. Do not attempt to open the lid and stop by hand or brake. What does labcorp test for in blood work? We use cookies to ensure that we give you the best experience on our website. The original Transpak containers can be used for these lock boxes. 4. Blood Tube Color Chart for specimens submitted to University of Colorado Hospital Laboratory GOLD or RED W/GELSERUM SEPARATOR (SST) GREEN WITH Do. Our online resources are here to support healthcare providers as well as patients. In work-up of pancreatitis, in addition to serum lipase and amylase, the 2-hour urine amylase is of value. These are easily identified by the yellow diagonal stripes on the label. Do not use oxalate, EDTA, or citrate plasma. WebSerum: red-top (no additive) tube; plasma or whole blood: gray-top (NaF/oxalate) tube, green-top (heparin) tube, or lavender-top (EDTA) tube. The essential difference between plasma and serum is that plasma retains fibrinogen (the clotting component), which is removed from serum. Send plasma in a plastic transport tube labeled Plasma, Sodium Fluoride. Send whole blood in a gray-top tube. Do I need to fast for a basic metabolic panel (BMP)? The LOINC codes are copyright 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. To facilitate making the vein more prominent, the patient may be asked to hold firmly to a rubber ball, a thick wad of gauze, etc. Immature granulocyte measurement using the Sysmex XE-2100. WebRed-top tube, gel-barrier tube, or green-top (lithium heparin) tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. In some cases, additional time should be Providers use a BNP or an NT-proBNP test to diagnose heart failure. What does it mean to cut a deck of tarot cards? (See Figure 2.) 0000516266 00000 n
(See Figure 15.). Inspect the serum for signs of hemolysis and turbidity by holding it up to the light. Get useful, helpful and relevant health + wellness information. Note: If a patient has intravenous (IV) solutions going into one or both arms, it is acceptable to puncture the vein 3 to 4 inches below the site of the IV. Centrifuge and transfer the plasma into a LabCorp PP transpak frozen biotin therapy for Multiple Sclerosis or oncology patients; skin, hair and nail supplements, or multivitamins containing > 5 mg/day) until at least 12 hours after the last biotin administration. The latest information about heart & vascular disorders, treatments, tests and prevention from the No. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD code chosen. Additional information regarding LOINC codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf. When more than one blood specimen is required, multiple sample needles and vacuum tubes make blood collection simpler and more efficient. The most common reason for delay in receiving results is inaccurate or out-of-date personal information on record with your health care providers or in your Labcorp Patient portal personal profile. The plastic BD PPT can be frozen at -80C prior to shipment.
B-type Natriuretic Peptide (BNP) Test - Cleveland Clinic 143000: NT-proBNP | Labcorp Spin specimen, separate plasma and send refrigerated. (See Figure 4.) With the syringe technique, venipuncture is accomplished without direct connection to the collection tube. Prolonged centrifugation may cause hemolysis. Slide preparation using venous blood is described below. Prolonged centrifugation may cause hemolysis. Blood cells are suspended in the plasma, which is made up of water and dissolved materials, including hormones, antibodies, and enzymes that are being carried to the tissues, and cellular waste products that are being carried to the lungs and kidneys. Following the collection, preparation, and transport instructions suggested by LabCorp supports the best possible test results. 2023 Laboratory Corporation of America Holdings. Douma RA, le Gal G, Shne M, et al. 12. Some laboratories offer a similar test called aminoterminal pro B-type natriuretic peptide (or NT-proBNP). These are easily identified by the yellow diagonal stripes on the label. 0000463973 00000 n
0.7 mL (Note: This volume does not allow for repeat testing.). Assessments of stained smears are performed if results meet specific numeric and/or instrument flagging criteria. Potential of an age adjusted D-dimer cutoff value to improve the exclusion of pulmonary embolism in older patients: A retrospective analysis of three large cohorts. If the glass syringe has been autoclaved, it should be oven dried before use. (Press Enter or Space to Go to Landing Page or Press Down to expand Menu), Blood Specimens: Chemistry and Hematology, Federally Qualified Health Centers (FQHCs), Combatting Modern Slavery and Human Trafficking Statement, Except when a blood alcohol is ordered, swab the site with a sterile alcohol sponge (70% alcohol) in a circular motion, inside to outside, to push contaminants away from the puncture site. Andrews PA, Thomas PA. Macro-amylasaemia as a cause of persistently raised serum amylase. An elevated percentage of IG has not been found to be clinically significant as a sole clinical predictor of disease. Presence of one or more of the following may be indication for further investigation: hemoglobin <10 g/dL, hemoglobin >18 g/dL, MCV >100 fL, MCV <80 fL, MCHC >37%, WBC >20,000/mm3, WBC <2000/mm3, presence of sickle cells, spherocytes, Pappenheimer bodies, basophilic stippling, stomatocytes, schistocytes (fragmented RBCs), target cells, oval macrocytes, teardrop red blood cells, abnormal cell populations, nucleated red blood cells in other than the newborn, blood parasites (malarial or Babesia organisms or the possibility of parasitic organisms), hypersegmented neutrophils, agranular neutrophils, hyposegmented neutrophils (Pelger-Hut anomaly or pseudo-Pelger-Hut [pelgeroid] cells), mononuclear cells in which apparent nucleoli are prominent (blast-like cells), presence of metamyelocytes, myelocytes, promyelocytes, neutropenia, presence of plasma cells, peculiar atypical lymphocytes, significant increase or decrease in platelets or bizarre platelets. 0000353809 00000 n
Always use the proper vacuum tube for tests requiring a special anticoagulant (eg, EDTA, heparin, sodium citrate, etc) or preservative. hb``pg``a``{ Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume. Use: Sodium citrate plasma. You can do this quickly while the patient's arm is elevated. This test monitors your heart rate through sensors attached to your skin. Blood cultures should be collected directly into the blood culture bottles provided by LabCorp. D-dimer levels can be elevated in many clinical circumstances, especially in hospitalized patients. A six-part differential reported in some lab locations includes IG % and IG absolute counts. What is the order of draw by tube color? The blood should not extend past 3/4 the length of the slide. If the arm is dry, you will avoid stinging the patient at venipuncture. ( Note: This volume does not allow for repeat testing.) Siemens Healthcare Diagnostics Products GmbH; 2010.
D-Dimer Place the tube(s) in a designated freezer. 0000039993 00000 n
The LOINC codes are copyright 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. 11. A BNP test is done by drawing blood from a vein in your arm using a hypodermic needle. Also, a clot tube (eg, red-top tube or gel-barrier) that does not clot should be brought to the attention of the physician. All Rights Reserved. Dempfle CE. Using just a small amount of blood, this test gives your provider important information about your heart health. Please refer to the specimen requirements for the test(s) of interest available in the Directory of Services. 14. 0000001776 00000 n
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0. A frozen specimen should be held in a freezer at 0C to -20C unless a specific test requires the specimen to be frozen at -70C (dry ice).
As soon as possible after collection, set the blood upright in a test tube rack. Attempts to force more blood into the tube by exerting pressure, as in collection with a syringe, will result in damage to the red cells (hemolysis). In the average adult male there are approximately 5 quarts (4.75 liters) of blood, composed of about 3 quarts (2.85 liters) of plasma and 2 quarts (1.9 liters) of cells. Do, Turn the centrifuge off, if not an automatic turn off, and allow it to come to a complete stop. Valli N, Georges A, Corcuff JB, et al. (Increasing the angle results in a thick film, while a smaller angle will produce a thin film.). Get the App Telemedicine Toolkit Let us help you navigate the new normal of virtual healthcare. 7. 0000000016 00000 n
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Centrifuge BD PPT/blood sample at room temperature at minimal of 1100 RCF (relative centrifugal force) for a minimum of 10 minutes in a swinging bucket rotor type centrifuge. BNP levels over 100 pg/mL may be a sign of heart failure. For more information, please view the literature below. Unless otherwise indicated, serum specimens may be sent at room temperature. Fernandes B, Hamaguchi Y. Do not apply fixative. Gel-barrier (mottled red/gray, gold, or cherry red-top) tubes contain clot activator and gel for separating serum from cells but include no anticoagulant. NT-proBNP levels may be lower in obese individuals. Puncture the diaphragm of the stopper by pushing the tube forward and initiating vacuum suction. Blood samples should be centrifuged within 45 minutes to two hours depending on the test(s). Plasma: 2 mL (Plasma Separator Tube (PST)), Plasma: 0.5 mL (Plasma Separator Tube (PST)). The major blood cells are classified as red cells (erythrocytes), white cells (leukocytes), and platelets (thrombocytes). 7 0 obj
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Adam SS, Key NS, Greenberg CS.
Pull the safety shield on the needle back over the holder before removing the needle shield. Turn the centrifuge off, if not automatic turn off, and allow it to come to a complete stop. Lab results are delivered to your Labcorp Patient portal account. After securing the tourniquet and reaffirming your selection of the best vein, both by sight and palpation, proceed as follows. 2021 Laboratory Corporation of America Holdings and Lexi-Comp Inc. All Rights Reserved. If blood is drawn for routine hematology, prepare the blood films (blood smears) immediately after collection. When frozen plasma is required, place plastic transport tube(s) immediately in the freezer compartment of the refrigerator, and notify your professional service representative that you have a frozen specimen to be picked up. Righini M, Van Es J, Den Exter PL, et al. The tube can be sent directly to the lab without transferring to a secondary tube. Electrochemiluminescent Immunoassay (ECLIA). 3. Materials for proper specimen collection and transport are supplied by LabCorp. Blood tests are very common and safe. Complete the test request form to indicate time and date of collection along with collector's identification.
BNP Test (B-type Natriuretic Peptide): Results and More - Healthline allowed for additional confirmatory or additional reflex tests. WebThis test, when ordered on Medicare patients, is subject to the National Coverage Determination (NCD) policies. 0000020185 00000 n
DVT clinical diagnosis should not be based on the result of Innovance D-dimer alone. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for
Lipase Several different types of anticoagulants interfere with the activity of these factors to prevent clotting. 10. Plasma is obtained from blood that has been mixed with an anticoagulant in the collection tube and has, therefore, not clotted. Some royal blue-top tubes do not contain EDTA. The needle may pinch or sting. Serum is usually collected in mottled red/gray, gold, or cherry red-top tubes, and red-top tubes are occasionally used. BNP is one of several proteins that help regulate blood circulation throughout your body. 23. 15. Permit the vacuum tube to fill completely. Additional information regarding LOINC codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf. (See Figure 7.). Enter the vein with the needle at an angle of approximately 15 to 30 degrees. A BNP test or NT-proBNP test detects heart failure by measuring the amount of BNP or its prohormone NT-proBNP in the bloodstream, respectively. Do not use oxalate, EDTA, or citrate plasma. Serum is obtained from clotted blood that has not been mixed with an anticoagulant (a chemical that prevents the clotting of blood). The distribution of the cells, as well as their morphology, can be altered by poor slide preparation. Also, never leave a tourniquet on the arm for more than one minute without releasing it. 5. When plasma is required, follow these steps. Follow these steps: There are two important guidelines to follow when submitting blood specimens. Stability Requirements Causes for Rejection Improper labeling Test Details Use In a healthy person, the white cells respond to minor infections by increasing in number and eliminating pathogens. McGlasson DL, More L, Best HA, Norris WL, Doe RH, Ray H. Drawing specimens for coagulation testing: Is a second tube necessary? Lipase activity is usually absent in urine, possibly from inactivation of the enzyme. Label the tube carefully and clearly with all pertinent information or bar code. This assay has been designed to minimize interference from heterophilic antibodies. 0000695552 00000 n
Centrifuge and transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N 49482). Turnaround time is defined as the usual number of days from the date of pickup of a specimen for Please verify that the diagnosis code (ICD code) you have chosen demonstrates medical necessity for the test as documented in the physicians patient record. 0000910226 00000 n
Heart failure medications, including beta blockers, ACE inhibitors and diuretics, can lower BNP or NT-proBNP levels in the blood. 0000806074 00000 n
2. Greasy or dirty slides, or use of a spreader slide with a chipped or unpolished end. The primary purpose of the white cells is to fight infection. 0000031049 00000 n
The BMP has 8 tests; the comprehensive metabolic panel (CMP) is a made up of 14 tests the 8 from the BMP as well as 2 protein tests (albumin and total protein) and 4 liver tests (ALP, ALT, AST, and bilirubin). The negative predictive value is further enhanced through the use of a clinical probability model along with D-dimer in the decision process.12-15 Values less than 0.5 mg/L FEU in an individual with a low clinical risk of venous thrombosis can serve as the basis for not performing more expensive diagnostic tests for DVT and PE. Select two clean, grease-free glass collection slides with frosted ends (new ones whenever possible). Serum must be separated from cells within 45 minutes to two hours depending on the test(s). Excessive bleeding (longer than five minutes) should be brought to the attention of the physician. A syringe is usually used with patients who are difficult to collect by routine venipuncture procedure, including techniques using a safety-winged blood collection set (butterfly). 2. Labs & Appointments Toggle Labs & Appointments, Billing & Insurance Toggle Billing & Insurance, Diseases & Conditions Toggle Diseases & Conditions, OnDemand Testing Toggle OnDemand Testing, Testing by Disease & Condition Toggle Testing by Disease & Condition, Testing & Services For Toggle Testing & Services For, Hospitals & Physician Systems Toggle Hospitals & Physician Systems, Managed Care Health Plans Toggle Managed Care Health Plans, Lab Data Integrations & Tools Toggle Lab Data Integrations & Tools, Employee Wellness & Testing Toggle Employee Wellness & Testing, Government & Education Toggle Government & Education, Therapeutic Indications Toggle Therapeutic Indications, Development Phase Toggle Development Phase, Compounds & Molecules Toggle Compounds & Molecules. ), 11. A separate frozen sample must be submitted for each test requiring a frozen specimen. Prepare the silver gel packs that fit into the Frozen Specimen Keeper by making sure that they also are frozen. (See Figure 6.) Break the paper seal on the needle cap in the presence of that patient, and remove the needle cap. 0000878985 00000 n
Gently tap tubes containing additives to dislodge any material that may be adhering to the stopper. Any delay will result in abnormal distribution of the white cells, with many of the larger white cells accumulating at the thin edge of the smear. Ordinarily, a 21- or 22-gauge needle is used. Use: Heparinized whole blood or plasma. You will likely need to fast for at least eight hours before your basic metabolic panel blood test. Note: Specimens to be tested by LabCorp should be collected in specimen containers provided by LabCorp. Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST , green, tan, yellow, pink, pearl, lavender. If only a single collection tube is required, when the vacuum is exhausted and the tube completely filled, release the tourniquet, and remove the tube from the needle assembly. Your provider only needs a small amount of blood for this test. 2. The CMS web site is available to assist you with this verification. Your provider will let you know when the results are ready. (Use of a fixed angle rotor centrifuge does not allow the gel barrier to form properly and may result in incomplete separation of plasma from the cellular components.). Use disposable plastic syringes and safety straight needles or a safety-winged blood collection set. BNP is released from the ventricles All Rights Reserved. This test gives your healthcare provider information about your heart.
140889: B-Type Natriuretic Peptide (BNP) | Labcorp Testing schedules may vary. Anchor the vein. Send plasma in a plastic transport tube labeled Plasma, Sodium Citrate. Send whole blood in a blue-top tube. 0000014084 00000 n
Serum lipase is usually normal in patients with elevated serum amylase, without pancreatitis, who have peptic ulcer, salivary adenitis, inflammatory bowel disease, intestinal obstruction, and macroamylasemia. 9500 Euclid Avenue, Cleveland, Ohio 44195 |, Important Updates + Notice of Vendor Data Event. Frozen Serum. Your provider removes some of your blood through a vein. 0000459642 00000 n
Olson JD, Cunningham MT, Higgins RA, Eby CS, Brandt JT. 0000016872 00000 n
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Examine tube to ensure that the gel barrier has formed between the plasma and the cellular elements. The chemical has been chosen to preserve some feature of the specimen and to work with the method used to perform the test. 4. After the test, your body naturally replenishes your blood supply. 19. 0000007790 00000 n
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Red-top tube, gel-barrier tube, or green-top (lithium heparin) tube. What is the difference between the BMP and CMP tests? 0000947383 00000 n
2. Your levels can also be lower if you have obesity or kidney failure. A lab examines your blood and checks the levels of BNP or NT-proBNP. Hold the spreader by the frosted end between the right thumb and the index finger. 0000520597 00000 n
When all samples have been drawn, remove the entire assembly from the arm. Jennersj CM, Fagerberg IH, Karlander SG, Lindahl TL. When frozen serum is required, place the plastic transport tube(s) (prepared above) immediately in the freezer compartment of the refrigerator.
It is recommended to ask all patients who may be indicated for this test about biotin supplementation. (Note: This volume does not allow for repeat testing.). Permit the vacuum tube to fill completely. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. 2. Refer to the. WebA B-type natriuretic peptide (BNP) test is a blood test. (See Figure 8.) A BMP may be ordered when you have a routine health exam. Utility of B-type natriuretic peptide in the diagnosis of congestive heart failure in an urgent-care setting. Electrolytes, serum calcium, glucose, and acetone are also often needed. Lowe GD. Put on the lab coat and gloves. allowed for additional confirmatory or additional reflex tests. Lipase isoforms and amylase isoenzymes: Assays and application in the diagnosis of acute pancreatitis. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. When multiple specimens are required, remove the first collection tube from the holder as soon as blood flow ceases, invert the first tube to prevent clotting, and gently insert the second tube into the holder. National Committee for Clinical Laboratory Standardization.